|
KMID : 0380020060210030224
|
|
Korean Journal of Biotechnology and Bioengineering
2006 Volume.21 No. 3 p.224 ~ p.229
|
|
|
Experimental and FEMLAB Simulation Study of Ibuprofen Racemate Separation in HPLC
|
|
Lee Eun
Kim Woo-Sik
Kim In-Ho
Chang Sang-Mork
Kim Jong-Min
|
|
|
Abstract
|
|
|
|
FEMLAB is a powerful interactive environment for modeling, solving all kinds of scientific and engineering problems based on partial differential equations (PDEs). Separation process of chiral compound in HPLC columns was simulated by FEMLAB. To study change of elution profile with isotherm models, non-competitive and competitive Langmuir adsorption isotherm were adopted. Separated material was (R, S)-ibuprofen [(R, S)-2-(4-isobutyl phenyl) propionic acid], an anti-inflammatory agent, which retain the pharmacological activity in the (S)-(+)-enantiomer. Sample concentrations were changed from 0.5 ㎎/㎖ to 2.0 ㎎/㎖ at a flow rate of 1 ㎖/min and flow rate varied from 1 ㎖/min to 3 ㎖/min at an ibuprofen concentration of 2.0 ㎎/㎖ and 20 ㎕ of injection volume. Simulated results were well fitted with experimental data.
|
|
|
KEYWORD
|
|
|
FEMLAB, Ibuprofen, HPLC, Langmuir isotherm
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|